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KMID : 0380019940090030319
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.319 ~ p.323
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The Studies for the Malate Tissue Biosensor Using Malate Dehydrogenase(Decarboxylatin) in the Bundle Sheath Cell of the Corn Leaf
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Hong Young-Seuk
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Abstract
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A biosensor for the measurement of malate has been constructed by the sodium-alginate immobilized bundle sheath cell tissue of corn leaf containing malate dehydrogenase(decarboxylating)(EC 1. 1. 1. 40) on the C02 gas-sensing electrode. The proposed tissue sensor had the linear in the range of malate concentration 5.6 x 10-,M-v2.5 x 10-´M with a slope of 53.5 mV/decade in 0.02M Tris-HCI buffer solution at optimum pH 8.0, and 25°C. A response time was 16. 18min. The present L-malate sensing tissue sensor is stable for more than one week. At pH 7.4, Km value was 0.6 x 10-´M. The various kinds of salt did not effect the signal of malate tissue biosensor as the inhibitor. We can measure the malate by the C02 electrode at the pH=8.0. Thus, the proposed tissue sensor will be useful for the measurement of malate.
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